A study investigated the effectiveness of ozone in inactivating respiratory syncytial virus (RSV), a leading cause of severe respiratory infections in infants and young children. Traditional disinfection methods using alcohol and detergents raise safety concerns in pediatric settings. The research utilized a dielectric barrier discharge (DBD)-based ozone generator, exposing the RSV A2 strain on glass coverslips to low-concentration ozone (0.5 ppm) for one hour under controlled conditions. Results showed that ozone exposure significantly decreased RSV infectivity, with viral titers reduced by more than 6 logs. Biochemical analyses indicated substantial reductions in intact RSV genomic RNA and F protein levels after ozone treatment, suggesting that ozone damages both the viral genome and surface proteins. These findings highlight the potential of the SFG1210 ozone generator as a practical, non-contact tool for surface disinfection of RSV, offering a safe approach for infection control in healthcare and childcare environments.