This study examines the facilitators, barriers, and recommendations for improving HIV self-testing (HIVST) uptake among men who have sex with men (MSM) and transgender (TG) individuals in Asia. Researchers conducted a systematic search of various databases and analyzed findings using the Confidence in the Evidence from Reviews of Qualitative Research (CERQual) approach. Results indicate that HIVST is viewed as a more confidential and accessible option than traditional HIV testing for MSM and TG populations. It has the potential to enhance the reach of HIV testing services. Community organizations and workers play a crucial role in facilitating HIVST among these groups. However, high costs for test kits present a significant barrier to uptake. Additionally, the availability of counseling services makes traditional testing more appealing. Many Asian countries lack supportive policies for HIVST among MSM and TG individuals. There are also concerns that HIVST may cause mental distress, leading to recommendations for integrating mental health services while ensuring user privacy.